Figure 10.

Murine ANT1, but not ANT2, is an ARL2·BART binding protein. Mitochondria were purified from the indicated tissues of wild-type (ant1⁺/ant1⁺) or ant1⁻/ant1⁻ mice and assayed by immunoblot and gel overlay analyses. Immunoblots were performed with ANT1 (top) or ANT2 (bottom) antibodies and the overlay assay used ARL2·[α-³²P]GTP·BART as probe. The lack of ANT1 immunoreactivity and activity in the binding assay are evident in the mitochondria from the knockout (KO) animal (right). These results are representative of multiple independent experiments.