Figure 12.

Amount of ARL2 in mitochondria from skeletal muscle and heart is markedly increased in animals deleted for ANT1. Mitochondria (top and bottom) and total soluble protein (S100; middle) from the indicated tissues were isolated from parental or ant1⁻/ant1⁻ mice and analyzed by immunoblotting (25 μg of protein/lane) by using ARL2 (top and middle) or mtHSP70 (bottom) antibodies. Differences in ARL2 levels inside mitochondria, compared with S100, are evident from comparisons of the top and middle panels. This experiment was repeated using tissues isolated from four separate pairs of control and knockout animals, with essentially identical results.