Figure 6.

ARL2 and BART behave as mitochondrial intermembrane proteins. Purified rat brain mitochondria (lane 1) were treated with digitonin at a protein:detergent ratio of 0.1 or 0.25 before separating soluble and particulate fractions, as described under MATERIALS AND METHODS. Immunoblots were performed as described previously, by using antibodies specific for mtHSP70 (a marker for matrix protein retention), cytochrome c (a marker for the inner membrane), ARL2, or BART. These results are representative of at least three separate experiments.