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. 2019 May 2;10:2041731419845852. doi: 10.1177/2041731419845852

Figure 3.

Figure 3.

(a) Proliferation of BM-hMSCs and AT-hMSCs cultivated in G-CH hydrogels measured by the CCK8 assay in the complete medium FBS or complete medium hPL at different time points (2, 6, 10, 14, and 21 days). Two-way ANOVA was used for statistical analysis. Data show the average values and their significant differences (*p ⩽ 0.05, **p ⩽ 0.01, ***p ⩽ 0.001, ****p ⩽ 0.0001). (b) Pictures of immunofluorescence of OCN expression by BM-hMSCs and AT-hMSCs cultured at 21 days in G-CH1 and G-CH2 hydrogels in the complete medium hPL (GM + hPL). Scale bar: 100 µm. (c) Graphs of immunofluorescence analysis of OCN expression by BM-hMSCs and AT-hMSCs cultured at 21 days in G-CH1 and G-CH2 hydrogels in the complete medium FBS (GM + FBS) or complete medium hPL (GM + hPL) or osteogenic medium FBS (OM + FBS) or osteogenic medium hPL (OM + hPL). One-way ANOVA was used for statistical analysis of the fluorescence intensity on BM-hMSCs and AT-hMSCs cultured at 21 days in G-CH1 and G-CH2 hydrogels.

Data show the average values and their significant differences (*p ⩽ 0.05, **p ⩽ 0.01, ***p ⩽ 0.001, ****p ⩽ 0.0001).