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. 2019 Apr 15;17(6):5821–5829. doi: 10.3892/ol.2019.10254

Figure 2.

Figure 2.

The expression of NSPc1 and lncRNAs in U87 cells subsequent to increasing or decreasing the expression of NSPc1. U87 cells were transfected with pCDEF-NSPc1 (overexpression plasmid) or pSE-NSPc1 (knockdown plasmid). (A) NSPc1 expression levels were determined using reverse transcription-quantitative polymerase chain reaction. GAPDH was used as the internal control. Results were analyzed using the 2−ΔΔCq method. (B) Protein expression levels of NSPc1 following transfection or knockdown of NSPc1 in U87 cells. GAPDH was used as an internal control. (C) lncRNA expression levels subsequent to regulation of NSPc1 expression with GAPDH as an internal control. Results were analyzed using the 2−ΔΔCq method. *P<0.05, **P<0.01 vs. control group. NSPc1, nervous system polycomb-1; lncRNAs, long non-coding RNAs; lncRNAs, long non-coding RNAs; MALAT1, metastasis associated lung adenocarcinoma transcript 1; ANRIL, antisense non-coding RNA in the INK4 locus; SOX2OT, sex-determining region of the Y chromosome-box 2 overlapping transcript; HOTAIR, homeobox protein transcript antisense RNA; APTR, alu-mediated CDKN1A/P21 transcriptional regulator; MEG3, maternally expressed 3; ADAMTS9-AS2, ADAMTS9 antisense RNA 2.