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. 2019 Apr 15;17(6):5821–5829. doi: 10.3892/ol.2019.10254

Figure 3.

Figure 3.

Expression of NSPc1 and lncRNAs in U87 cells following ATRA-induced differentiation. (A) Measurement of the expression levels of NSPc1 by reverse transcription-quantitative polymerase chain reaction following ATRA-induced differentiation of U87 cells. GAPDH was used as an internal control. Results were analyzed using the 2−ΔΔCq method. (B) Protein expression levels of NSPc1 following ATRA-induced differentiation of U87 cell by western blotting. GAPDH was used as an internal control. (C) Expression of lncRNAs following ATRA-induced differentiation of U87 cells. GAPDH was used as the internal control. Results were analyzed using the 2−ΔΔCq method. *P<0.05, **P<0.01. NSPc1, nervous system polycomb-1; lncRNAs, long non-coding RNAs; ATRA, all-trans retinoic acid; MALAT1, metastasis associated lung adenocarcinoma transcript 1; ANRIL, antisense non-coding RNA in the INK4 locus; SOX2OT, sex-determining region of the Y chromosome-box 2 overlapping transcript; HOTAIR, homeobox protein transcript antisense RNA; APTR, alu-mediated CDKN1A/P21 transcriptional regulator; MEG3, maternally expressed 3; ADAMTS9-AS2, ADAMTS9 antisense RNA 2.