Figure 4.

HMGB1 is targeted by miR-129-5p in A549 cells. (A) The binding sites and its mutated version of HMGB1 and miR-129-5p. (B) miR-129-5p mimic and plasmid containing WT or mutated 3′-UTR of HMGB1 were transfected into A549 cells. After 48 h, the luciferase activity was measured. (C) The miR-129-5p mimic was transfected into A549 cells, and the abundance of the HMGB1 protein was analyzed by western blotting. The experiment was performed at least three times. *P<0.05 vs. WT. HMGB1, high mobility group box 1; UTR, untranslated region; WT, wild-type; NC, negative control; miR, microRNA.