Skip to main content
. 2019 Apr 18;17(6):5839–5840. doi: 10.3892/ol.2019.10268

Figure 2.

Figure 2.

COX 2 inhibition increases the sensitivity of lung cancer cells to X-ray radiation. (A) Total RNA extract was amplified by reverse transcription-polymerase chain reaction and separated by agarose gel electrophoresis. Upper and lower lanes are COX-2 and β-actin, respectively. (B) Nuclear morphology of A549 cells treated with DMSO, X-rays, celecoxib or celecoxib plus X-rays. Nucleic changes, including anachromasis, pyknosis and karyorrhexis are indicated by the white arrows. Nuclei were stained with Hoechst 33258; image magnification, ×400. (C) A549 cells were irradiated with various doses of X-ray radiation, then treated with/without 200 μM celecoxib for three days. The growth inhibition curve is presented. Data are presented as the mean and standard error of five replicates. ****P<0.0001, two-way ANOVA. (D) Growth inhibition of A549 cells under indicated treatment. Data were pooled from three parallels and are presented as the mean ± standard deviation. Survival ratio at day 5 was subjected to statistical analysis. ****P<0.0001, two-way ANOVA. COX-2, cyclooxygenase-2; DMSO, dimethyl sulfoxide; ANOVA, analysis of variance.