Figure 4. Male Bdnf^lox/lox;Emx1^Cre/+ mice were resistant to DIO within the thermoneutral zone.

(A) Daily intake of chow diet and HFD at the thermoneutral temperature (30°C). N=6-8 mice per group; ns, no significance by Student’s t test.

(B) Body weight of male mice housed at thermoneutrality. Eight-week-old mice were switched to thermoneutrality from standard housing. They were maintained on chow diet until 16 weeks of age when they started receiving HFD for 12 weeks. N=6-8 mice per group; Two-way ANOVA followed by Bonferroni’s test: genotype, F_(1,240)=689, p<0.0001; ***p<0.001 and ****p<0.0001 when comparisons were made at individual time points.

(C) Weight of individual fat tissues after HFD feeding for 12 weeks at thermoneutrality. N=6-8 mice per group; **p<0.01 by Student’s t test.

(D) IPGTT in mice fed HFD for 10-11 weeks at thermoneutrality. N=6-8 mice per group; Two-way ANOVA followed by Bonferroni’s test: genotype, F_(1,72)=37.61, p<0.0001; **p<0.01 and ***p<0.001 when comparisons were made at individual time points.

(E) IPITT in mice fed HFD for 10-11 weeks at thermoneutrality. N=6-8 mice per group; Two-way ANOVA followed by Bonferroni’s test: genotype, F_(1,51)=52.18, p<0.0001; **p<0.01 when comparisons were made at individual time points.

(F) O₂ consumption of mice at thermoneutrality during the first week of HFD feeding. N=6-8 mice per group; Two-way ANOVA followed by Bonferroni’s test: genotype, F_(1,288)=189.5, p<0.0001; *p<0.05, **p<0.01, ***p<0.001 and ****p<0.0001 when comparisons were made at individual time points.

(G) Body temperature of mice housed at thermoneutrality. N=6-8 mice per group; **p < 0.01 by Student’s t test.

(H) Concentration of norepinephrine in the plasma of HFD-fed mice. N=8-10 mice per group; *p<0.05 by Student’s t test.

All data were presented as mean ± SEM.