KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-UCP1 | Abcam | Cat# ab10983 RRID:AB_2241462 |
| Mouse monoclonal anti-GAD65 | Abcam | Cat# ab26113, RRID:AB_448989 |
| Rabbit polyclonal anti-UCP1 | ThermoFisher Scientific | Cat# PA1-24894, RRID:AB_2241459 |
| Rabbit polyclonal anti-HSL | Cell Signaling Technology | Cat# 4107 RRID:AB_2296900 |
| Rabbit polyclonal anti-phospho-HSL (Ser563) | Cell Signaling Technology | Cat# 4139, RRID:AB_2135495 |
| Mouse monoclonal anti-Myc-Tag (9B11) | Cell Signaling Technology | Cat# 2276, RRID:AB_331783 |
| Mouse monoclonal anti-β-Actin | Sigma-Aldrich | Cat# A5441, RRID:AB_476744 |
| Mouse monoclonal anti-α-Tubulin | Sigma-Aldrich | Cat# T8203, RRID:AB_1841230 |
| Rabbit polyclonal anti-Tyrosine Hydroxylase | Sigma-Aldrich | Cat# AB152, RRID:AB_390204 |
| Rabbit polyclonal anti-β-galactosidase | Cappel/ICN Pharmaceuticals | Cat# 55976, RRID:AB_2313707 |
| Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP | ThermoFisher Scientific | Cat# A16110, RRID:AB_2534782 |
| Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP | ThermoFisher Scientific | Cat# 31430, RRID:AB_228307 |
| Alexa Fluor® 594 AffiniPure Goat anti-Rabbit IgG, F(ab’)2 fragment specific | Jackson ImmunoResearch Labs | Cat# 111-585-006, RRID:AB_2338060 |
| Biotinylated Goat anti-Rabbit IgG Antibody | Vector Laboratories | Cat# BA-1000, RRID:AB_2313606 |
| Bacterial and Virus Strains | ||
| AAV-GFP (AAV2-CMV-GFP) | University of North Carolina’s Vector Core | N/A |
| AAV-Cre-GFP (AAV2-CMV-Cre-GFP) | University of North Carolina’s Vector Core | N/A |
| AAV-BDNF (AAV2-CMV-BDNF-Myc) | This paper | N/A |
| PRV152 | NIH Center for Neuroanatomy with Neurotropic Viruses | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| β-Carboline-3-carboxylic acid N-methylamide (FG7142) | Sigma-Aldrich | Cat# E006-100MG |
| 7-bromo-5-(2-pyridyl)-3H-1,4-benzodiazepin-2(1H)-one (bromazepam) | Sigma-Aldrich | Cat# B4144-100MG |
| Insulin (Humulin) (IPITT) | Eli Lilly | Cat# VI7510 |
| CNQX | Tocris | Cat# 0190 |
| DL-APV | Sigma-Aldrich | Cat# A5282-10MG |
| Tetrodotoxin (TTX) | American Radiolabeled Chemicals | Cat# 50-753-2807 |
| Tween 80 | Sigma-Aldrich | Cat# P1754-25ML |
| Trizo | ThermoFisher Scientific | Cat# 15596018 |
| D-(+)-Glucose (IPGTT) | Sigma-Aldrich | Cat# G8270-1kg |
| FastStart Universal SYBR Green Master (Rox) | Roche | Cat# 4913914001 |
| Critical Commercial Assays | ||
| Norepinephrine ELISA Kit | Abnova | Cat# KA1891 |
| Corticosterone ELISA kit | Enzo Life Sciences | Cat# ADI-900-097 |
| Deposited Data | ||
| Raw and analyzed data | This paper | |
| Experimental Models: Organisms/Strains | ||
| C57BL/6J WT mice | The Jackson Laboratory | JAX: 000664 |
| Bdnflox/+ mice | The Jackson Laboratory | JAX: 004339 |
| Emx1Cre/+ mice | The Jackson Laboratory | JAX: 005628 |
| KA1-Cre mice | The Jackson Laboratory | JAX: 006474 |
| Bdnfklox/+ mice | The Jackson Laboratory | JAX: 021055 |
| BdnfLacZ/+ mice | Liao et al., 2012 | N/A |
| Oligonucleotides | ||
| A full list of Primers is in Table S1 | This paper | N/A |
| Software and Algorithms | ||
| Clampex 10.6 | Molecular devices | https://www.moleculardevices.com |
| Clampfix 10.6 | Molecular devices | https://www.moleculardevices.com |
| GraphPad Prism7 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| ImageJ bundled with Java 1.8.0_172 | NIH ImageJ | https://imagej.nih.gov/ij/ |
| EthoVision XT 11.5 | Noldus | https://www.noldus.com/animal-behavior-research/products/ethovision-xt |
| Adobe CS6 | Adobe | https://www.adobe.com |
| Excel (2011) | Microsoft | https://www.microsoft.com/enus/download/office.aspx |
| Stepone software | Applied Biosystems | https://www.thermofisher.com/us/en/home/technical-resources/softwaredownloads/StepOne-and-StepOnePlus-Real-Time-PCR-System.html |
| NIS-Elements software | Nikon | https://www.nikoninstruments.com/Products/Software |
| Other | ||
| Regular Chow Diet | Harlan Teklad | Cat# 2920× |
| High Fat Diet | Research Diets | Cat# D12492 |