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. 2019 Mar 19;17(6):4811–4818. doi: 10.3892/ol.2019.10164

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Copyright: © Kawashima et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Association of CD151 with MMP-9 expression in representative gelatin zymograms of culture supernatants of siRNA-mediated CD151 silencing and control siRNA-treated HS-MM^high cells. (A) MMP-9 active band was weakly evident in CD151-downregulated HS-MM^high cells (indicated by siCD151) compared with control cells without siRNA-treatment or with control siRNA (indicated by siMock). (B) MMP-9 was reduced by the downregulation of CD151. The results shown were obtained using SI02777257 siRNA (Qiagen) to silence the CD151 gene. Similar results were obtained using SI02777250 siRNA. The data represent mean ± SD of triplicate assays. ANOVA and Tukey's test was performed to confirm the significant differences, **P<0.01 vs. si-Mock group. CD151, cluster of differentiation 151; MMP-9, matrix metalloproteinase-9; SD, standard deviation; siRNA, small interfering RNA; MMP, matrix metallopeptidase.