Extended Data Fig. 4. Deletion of NR4A1 in T cells results in overexpression of IL-2 and IFN γ.

a, qRT–PCR measurement of expression of Nr4a1, Il2 and Ifng in wild-type and Nr4a1^−/− CD4⁺ and CD8⁺ T cells stimulated with plate-coated anti-CD3 and anti-CD28 at indicated time points. b , Flow cytometry analysis of IFNγ and IL-17A expression in wild-type and Nr4a1^{−/ −} CD4⁺ and CD8⁺ T cells from lymph nodes (LN) and spleens (SP). c, ELISA measurement of IFNγ and IL-2 expression in splenocytes from wild-type and Nr4a1^{−/ −} mice in the context of food oral tolerance. PBS-treated mice were used as a control group. *P < 0.05. OD, optical density. d, Equal amounts of CD45.1⁺ wild-type and CD45.2⁺Nr4a1^−/− bone marrow cells (4 × 10⁶ per mouse) were transferred into Rag1^−/− mice. Flow cytometry analysis of CD4⁺FOXP3⁺ T_reg cells in thymus, spleen and lymph nodes (LN) from chimeric mice two months after reconstitution. Six-week-old mice; n = 3 per group (a–c); n = 4 per group (d). P values were calculated using a two-sided unpaired Student’s t-test. N.S., not significant. All data are representative of two independent experiments and graphs show mean ± s.d.