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. Author manuscript; available in PMC: 2020 Mar 19.
Published in final edited form as: Immunity. 2019 Mar 19;50(3):655–667.e4. doi: 10.1016/j.immuni.2019.02.013

Figure 3. Lgr5-derived hair follicle stem cells contribute to epidermal repair.

Figure 3

(a) Diagram of hair follicle anatomy. Following epidermal injury, cells derived from HFSCs located in the bulge region migrate into the upper hair follicle (isthmus and infundibulum) and interfollicular epidermis (IFE) to participate in epithelial repair.

(b) Schematic of Lgr5-tdTom mice. Lgr5 drives expression of eGFP and a tamoxifen inducible Cre (CreERT2). These mice are crossed to R26-tdTomato mice. Injection with tamoxifen allows for inducible and permanent labeling of Lgr5+ stem cells and their progeny.

(c) The back skin of Lgr5-tdTom mice were injured (as in Supplementary Figure 1a) and injected with tamoxifen on day 0. Mice were harvested on days 2,4 and 7 of recovery and compared to uninjured Lgr5-traced mice.

(d) Representative high-power images of Lgr5-traced cells in the bulge, upper hair follicle and IFE during barrier regeneration compared to uninjured control.

(e) Quantification of Lgr5-derived cells in the IFE during epidermal repair. Error bars are +/− S.E.M. n.s.- no significance; **p < 0.01; *** p<0.001. n=2–4 mice per group. IFE – Interfollicular epidermis; Infund. – Infundibulum.