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. 2019 Apr 9;17(6):5343–5350. doi: 10.3892/ol.2019.10235

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — SIRT1 knockdown inhibits glioma cell proliferation. (A) SIRT1 was silenced by shRNA in U251 cells. (B) SIRT1 was silenced by shRNA in U87 cells. (C) Growth curve of U87 cells as detected by WST-1 assay. (D) Growth curve of U251 cells as detected by WST-1 assay. NC, negative control; OD, optical density; shRNA, short hairpin RNA; shSIRT1, short hairpin RNA sirtuin 1; SIRT1, sirtuin 1; WST-1, water soluble tetrazolium salts 1. All determination assays were carried out in triplicate.