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. 2019 Apr 17;17(6):5747–5753. doi: 10.3892/ol.2019.10262

Figure 2.

Figure 2.

CA inhibits VEGFR2 kinase activity. (A) A549 cells were treated with beads only (control) or increasing concentrations of CA (0, 4 and 8 µM) for 24 h, prior to co-IP with anti-VEGFR2 antibody, and subsequent western blotting with anti-phospho-Serine antibody. Total VEGFR2 and GAPDH were detected in lysates (Input). (B) Quantification of phosphor-VEGFR2 level shown in (A), normalized by total VEGFR2. (C) CA effects on VEGFR2 kinase activity measured by ADP-Glo™ kinase assay (n=4; kinase activity data were normalized to the control group and the results are shown as percentages). (D) A549 cell were treated with SU1498 for 24 h and with indicated concentrations of CA for another 24 h. Cell migration ability was measured by Transwell assay. n=4. *P<0.05 and ***P<0.001, compared with the 0 µM group. CA, corosolic acid; VEGRF2, vascular endothelial growth factor receptor 2; IP, immunoprecipitation.