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. 2019 Mar 22;17(6):5201–5209. doi: 10.3892/ol.2019.10186

Figure 6.

Figure 6.

Knockdown of ANXA1 can reverse the inhibitory effects of miR-196a downregulation on the proliferation, invasion and migration of EC109 cells. (A) Protein expression levels of ANXA1 in EC109 cells were measured by western blotting. β-actin was used as a control. The results demonstrated that ANXA1 protein expression was significantly upregulated by miR-196a inhibitor and markedly downregulated by ANXA1 siRNA. (B) Proliferation of EC109 cells was detected by MTT assay. The miR-196a inhibitor suppressed the proliferation of EC109 cells, whereas co-transfection with ANXA1 siRNA reversed this inhibitory effect. (C) Invasive and (D) migratory abilities of EC109 cells were determined by Transwell cell invasion and migration assays. Magnification, ×200. Downregulation of miR-196a significantly suppressed the invasion and migration of EC109 cells, whereas knockdown of ANXA1 reversed these inhibitory effects. Experiments were performed in triplicate and data are expressed as the means ± standard deviation. *P<0.05, **P<0.01 vs. inhibitor NC; ##P<0.01, as indicated. ANXA1, Annexin A1; miR-196a, microRNA-196a; NC, negative control; siRNA, small interfering RNA.