Table 2.
Primer | Sequence (5′-3′) | Product size (bp) | PCR cycles | Annealing temperature (°C) |
---|---|---|---|---|
c-MYC-F | CGCAAATGGGCGGTAGGCGTG | 165 | 40 | 60 |
c-MYC -R | TAGAAGGCACAGTCGAGG3 | |||
Ecad-F | TACACTGCCCAGGAGCCAGA | 103 | 40 | 60 |
Ecad-R | TGGCACCAGTGTCCGGATTA | |||
Vimentin-F | GACCAGCTAACCAACGACAA | 150 | 40 | 60 |
Vimentin-R | GTCAACATCCTGTCTGAAAGAT | |||
Ncad-F | CGAATGGATGAAAGACCCATCC | 174 | 40 | 60 |
Ncad-R | GGAGCCACTGCCTTCATAGTCAA | |||
Linc00152-F | TGAGAATGAAGGCTGAGGTGT | 116 | 40 | 60 |
Linc00152-R | GCAGCGACCATCCAGTCATT | |||
GAPDH-F | GGAGTCAACGGATTTGGT | 206 | 40 | 60 |
GAPDH-R | GTGATGGGATTTCCATTGAT |
F – forward; R – reverse.