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. 2019 May 1;25:3199–3211. doi: 10.12659/MSM.915417

Figure 7.

Figure 7

Propofol suppresses HMGB1 production by mechanical stretch in vivo and in vitro. Animals were put on LVT (6 ml/kg) or HVT (30 ml/kg) ventilation for 4 h. Propofol (10 mg/kg) was introduced prior to onset of ventilation, followed by infusion at 5 mg/(kg·h). HMGB1 mRNA expression (A) in mouse pulmonary tissue was determined by qPCR. HMGB1 protein expression in mouse pulmonary tissues (B) and BAL fluid (C) was estimated by ELISA assay (n=7). Primary cultured MLVECs were exposed to cyclic stretch in presence or absence of propofol (100 μM) for 4 h. HMGB1 protein levels in cell culture supernatant (D) were determined by ELISA assay (n=4). Results are displayed as mean ±SEM. ** p<0.01 vs. Control; ## p<0.01 vs. 30 ml/kg ventilation; # p<0.05 compared to cyclic stretch.