Table 1.

Effect of Tf recycling in cells microinjected with antibodies specifically against Glu tubulin and Tyr tubulin peptides

	% of cells showing Cy3-Tf retention in the ERC
Anti-Try antibody (W2 injected)	12 (n = 203)
Anti-Glu antibody (SG injected)	59 (n = 197)
Control (no injection)	5 (n = 327)

Data are the percentage of the control or the microinjected cells (n) exhibiting retention of Cy3-Tf in ERC after 1-h chase. TRVb-1 cells were incubated with Cy3-Tf for 1 h before the microinjection of the anti-Tyr or anti-Glu antibodies as described in MATERIALS AND METHODS. Cells were then washed and chased in the absence of Cy3-Tf but in the presence of excess unlabeled Tf and desferroxamine for 1 h.