Figure 5.

Infection with L. braziliensis increases the frequency of TLR2 ⁽⁺⁾ and TLR4 ⁽⁺⁾ monocytes expressing TNF and IL-10. PBMC-derived monocytes from CL patients (n = 9) were infected with L. braziliensis (ratio 5:1) for 8 h. The data represent the frequency of TLR2 ⁽⁺⁾ and TLR4 ⁽⁺⁾ on monocytes (CD14+) from CL patients (n = 9) expressing TNF (A) and IL-10 (B). The percentage of CD14+ cells expressing cytokines in non-infected and infected cells was determined by gating on the corresponding population. The results are expressed as median and Mann-Whitney test was used for statistical analyses (^*P < 0.05, ^**P < 0.01, ^***P < 0.001).