Figure 6.

Monocytes from CL patients expressing TLR2 and TLR4 produce more TNF and IL-10 after L.braziliensis infection. PBMC-derived monocytes from CL patients (n = 9) were infected with L.braziliensis (ratio 5:1) for 8 h. The data represent the frequency of cells expressing TNF (A) and IL-10 (B) on monocytes TLR⁽⁺⁾ or TLR⁽⁻⁾. The frequency of CD14+ cells expressing cytokines on cells TLR⁽⁺⁾ or TLR⁽⁻⁾ was determined by gating on the corresponding population. The results are expressed as median and Mann-Whitney test was used for statistical analyses (^*P < 0.05, ^**P < 0.01, ^***P < 0.001).