Figure 7.

Frequency of monocytes subsets from CL patients expressing TNF and IL-10 in cells TLR2 ⁽⁺⁾ and TLR4 ⁽⁺⁾ after L. braziliensis infection. PBMC-derived monocytes from CL patients (n = 9) were infected with L. braziliensis (ratio 5:1) and stained with CFSE for 8 h. The data represent the frequency of cells expressing TNF (A) and IL-10 (B) on classical (CD14^highCD16⁻), intermediate (CD14^highCD16⁺), and non-classical monocytes (CD14^lowCD16⁺) after infection with L. braziliensis. The percentage of monocytes expressing cytokines on cells TLR ⁽⁺⁾ was determined by gating on the corresponding population. The results are expressed as median. Kruskal-Wallis with Dunn's post-test were used for statistical analyses (^*P < 0.05, ^**P < 0.01).