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Western analysis of affinity-purified antibodies against AspB. (a) Total protein from A. nidulans strain A850 stained with Coomassie blue. Blots of total protein were incubated with 1:100 dilutions of unpurified immune serum (b), antibodies obtained after one round of affinity purification (c), or antibodies obtained after two rounds of affinity purification (d). A single 49-kDa polypeptide is recognized (arrow).
