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Dependence of AspB localization on mitosis. DIC images (left column). Chitin and nuclear staining with Calcofluor and Hoechst (middle column). AspB localization (right column). (a–c) bimE7 mutant grown at 30°C for 10 h and then shifted to 42°C for 2 h. No AspB localization was detected. (d–f) bimE7 mutant grown at 30°C for 10 h, shifted to 42°C for 2 h, and shifted back to 30°C for 45 min. AspB localization was detected at septation sites.
