Conditional mutant of aspB. (a, c,
and e) DIC images of vegetative hyphae. (b, d, and f) Chitin and
nuclear staining of vegetative hyphae with Calcofluor and Hoechst. (a
and b) Wild-type A. nidulans grown at 42°C for 10
h. (c–f) A. nidulans strain APW55
(aspB-318) grown at 42°C for 10 h. Note that
chitin staining of septa (arrows) appears weaker than in wild type.
(g–j) DIC images of conidiophores exposed to an air interface for
24 h. (g) Wild-type conidiophore. (h–j) APW55 conidiophores. Bar,
5 μm.