Figure 3.

GBF1 and BIG1 localize to cis- and trans-compartments of the Golgi complex, respectively. NRK cells were fixed and processed for double-label IF by using either polyclonal anti-TGN38 (a), anti-GBF1 (d), or anti-BIG1 (g), and monoclonal anti-p115 (c, f, and i), or the two polyclonal antibodies Alexa488-conjugated 9D3 (anti-BIG1, j) and anti-TGN38 (l). Shown are single slice confocal images. Middle panels (b, e, h, and k) show merged left and right images. The inset shows a threefold magnification of the area indicated by an arrowhead. Bar, 10 μm. Shown are representative data from at least three experiments with similar results.