Figure 4.

N terminal fragment of BIG2, like BIG1, does not colocalize with GBF1 and the cis-Golgi marker p58. BHK-21 cells were transiently transfected with a plasmid encoding HA-tagged N- BIG2 and processed for double-label IF by using monoclonal anti-HA and polyclonal antibodies against either p58 (a–c) or GBF1 (d–f). Shown are single slice confocal images obtained with the indicated antibody. Middle panels (b and e) show superimposed left and right images. The inset shows a threefold magnification of the area indicated by an arrowhead. Bars, 5 μm. Distinct staining for HA-BIG2 and GBF1 was observed in each of four experiments.