Figure 5.

GBF1 and BIG1 redistribute to distinct structures in response to BFA. NRK cells were treated with either 0.1% DMSO (control) or 10 μg/ml BFA for 30 min at 37°C before fixation and processing for IF. Cells were either double stained using two polyclonal antibodies (a–d) against GBF1 (H154/Alexa594; a and c) and BIG1 (Alexa488-conjugated 9D3; b and d) or singly stained using polyclonal antibodies against Man II (e) or TGN38 (f). Shown are single-slice confocal images. Bar, 10 μm. Similar results were obtained from at least two independent experiments.