Figure 8.

GBF1 and BIG1 overlap with distinct sets of coat proteins: GBF1 significantly with β-COP and BIG1 preferentially with clathrin. NRK cells were fixed and processed for double-label IF by using anti-β-COP and anti-GBF1 (a–c), anti-β-COP and anti-BIG1 (d–f), anti- clathrin and anti-GBF1 (g–i), or anti-clathrin and anti- BIG1 (j–l). Shown are single-slice confocal images taken in the indicated channel. Middle panels (b, e, h, and k) show superimposed left and right images. The inset shows a fourfold magnification of the area indicated by arrowhead. Arrows in b, c, e, and f point to some peripheral structures stained by anti-β-COP only. Bars, 5 μm. Shown are representative data from at least two experiments.