Fig. 2.

IL-2/antibody complex increases the number of Th2 cells and type-2 innate lymphoid cells despite the increase in the number of Foxp3⁺ regulatory T cells. Allergic airway inflammation was induced as described in Fig. 1. (A) Frequencies of Foxp3⁺ cells gated on CD4⁺ T cells (left) and numbers of Foxp3⁺ CD4⁺ T cells (right) in the lung from indicated mice. (B) Representative FACS plots of IFN-γ and IL-13. (C) Frequencies of IL-13⁺ cells gated on CD4⁺ T cells (left) and number of IL-13⁺ CD4⁺ T cells (right) in the lung. (D) Representative FACS plots of GATA3 and RORγt gated on Lin⁻ Thy1⁺ cells. (E) Frequencies of innate lymphoid cell (ILC) subsets; ILC1, type-1 (GATA3⁻, RORγt⁻); ILC2, type-2 (GATA3⁺, RORγt⁻); ILC3 type-3 (GATA3⁻, RORγt⁺) (left) and numbers of innate lymphoid cell subsets (right). Numbers in parentheses are fold-difference between OVA/Alum and OVA/Alum+IL-2C. Data are pooled from two independent experiments (n = 6–8). Error bars denote mean ± S.E.M; **P < 0.01, ***P < 0.001.