Fig. 3.

IL-2/antibody complex alleviates Th17-mediated airway inflammation. C57BL/6 mice were i.n.-sensitized with OVA or PBS plus LPS and then i.n.-challenged with OVA. During OVA-challenge, OVA-sensitized mice were i.p.-treated with PBS or IL-2C once daily for three consecutive days. (A) Frequencies of Foxp3⁺ cells gated on CD4⁺ T cells (left) and numbers of Foxp3⁺ CD4⁺ T cells (right) in the lung from indicated mice. (B) Representative lung section stained with hematoxylin and eosin (scale bar, 100 μm). (C) Histological scores. (D) Representative FACS plots of IFN-γ and IL-17 gated on CD4⁺ T cells in the lung. (E) Frequencies of IFN-γ⁺ (left) and IL-17⁺ cells (right) gated on CD4⁺ T cells in the lung. (F) Numbers of IFN-γ⁺ (left) and IL-17⁺ CD4⁺ T cells (right). Data are pooled from two independent experiments (n = 6–8). Error bars denote mean ± S.E.M; *P < 0.05, **P < 0.01, ***P < 0.001. ns, not significant.