Fig. 4.

IL-2-mediated signaling is required for the induction of Th2 cells. (A) Representative FACS plots showing the frequencies of Foxp3⁺ cells and effector T cells (CD44^hi Foxp3⁻) gated on CD4⁺ T cells in spleen (SPL) from wild-type (WT) and IL-2^−/− mice. (B) Representative histogram showing GATA3 expression (left) and mean fluorescence intensity (MFI) of GATA3 expression gated on CD4⁺ T cells in SPL from WT and IL-2^−/− mice. (C–E) Foxp3-DTR mice were treated with PBS or diphtheria toxin (DT). DT-treated mice were injected with PBS or anti-IL-2 depleting antibody. Cytokine production from CD4⁺ T cells in SPL was examined. (C) Representative FACS plots of IFN-γ and IL-13 gated on CD4⁺ T cells in the SPL from indicated mice. (D) Frequencies of IFN-γ⁺ (left) or IL-13⁺ (right) cells gated on CD4⁺ T cells. (E) Number of IFN-γ⁺ (left) and IL-13⁺ CD4⁺ T cells (right). Data represent one of two independent experiments (n = 3–4). Error bars denote mean ± S.E.M; *P < 0.05, **P < 0.01, ***P < 0.001. ns, not significant.