Skip to main content
. 2019 Apr 5;160(6):1377–1393. doi: 10.1210/en.2018-00731

Figure 7.

Figure 7.

Nuclear NR2F2 and AR were present in VCAM1+ PCOS theca cells; the AR antagonist flutamide markedly and selectively reduced VCAM1 mRNA. (A, B) IF showed that NR2F2 was expressed and nuclear (38) in control and PCOS theca cells in culture. (C, D) IF also showed that AR was nuclear (35) and was colocalized with VCAM1+ theca cells where VCAM1 appears membrane bound (31). (E) PCOS patients expressed higher levels of VCAM1 mRNA than did control patients, whereas AR and NR2F2 mRNA levels were similar in control and PCOS samples. (F) Exposure of PCOS theca cells to a dose-dependent increase of the AR antagonist flutamide markedly reduced expression of VCAM1 mRNA and increased NR2F2 without changes in AR transcript level. N = 3 for all conditions. **P < 0.025, ***P < 0.01 by Student t test.