Figure 2.

Components of the 48S preinitiation complex are recruited to stress granules. DU145 cells were treated with arsenite (0.5 mM, 1 h) and processed for multicolor immunofluorescence microscopy by using antibodies reactive with TIA-1/R (green), the indicated initiation factor or other protein (red), and DNA (blue). Each vertical column shows views of the same field. Top row: TIA-1 or TIAR (green). Middle row (red): eIF2α (A), eIF3 (B), eIF4E (C), eIF4G (D), and eIF5 (E). Bottom row, merged views. Yellow arrows point out individual stress granules in which both the red and the green signals overlap; white arrowheads indicate the position of SGs that only contain TIA. Nuclei are stained blue. Bar, 20 μm.