Figure 5.

Kinetics of the colocalization of TIAR and eIF3 at stress granules. DU145 cells were treated with 1.0 mM sodium arsenite for 30 min, washed, and then further incubated in the absence of arsenite (recovery) for the indicated times. Each row depicts parallel views of the same field. TIA-1/R appears green, eIF3 appears red, and coincidence appears yellow (right column: merge). Control (A), 30-min arsenite treatment without recovery (B), 30-min arsenite followed by 30-min recovery (C); 30-min arsenite followed by 1-h recovery (D); 30-min arsenite followed by 2-h recovery (E); and 30-min arsenite followed by 3-h recovery (F). Nuclei are stained blue. Bar, 20 μm.