Figure 2.

SA‐binding activities of PP2Cs are enhanced by ABA and this binding is partially suppressed by SA. (a–c) Sensorgrams obtained with recombinant, purified 1 μM of His₆‐tagged PP2C‐D4 (a), ABI1 (b), and ABI2 (c) using a 3AESA‐immobilized sensor chip in the absence or in the presence of 2 mM ABA or 2 mM ABA plus 2.5 mM SA. Signals detected from a mock‐coupled control chip were subtracted. The experiments were independently repeated at least twice