Figure 5.

SA suppresses ABA‐induced gene expression. (a) Transcript levels, as measured by qRT‐PCR, in seedlings pretreated with either water, 100 μM ABA, 100 μM SA, or 100 μM ABA plus 100 μM SA. Transcript levels of PP2C‐D4, ABI1, and ABI2 were determined at 3 hr post‐treatment (hpt). Data are averaged ±SD (n = 3). (b) Transcript levels as measured by qRT‐PCR of ABA‐responsive marker genes in seedlings pretreated with either water, 100 μM ABA, 100 μM SA, or 100 μM ABA plus 100 μM SA. Transcript levels of RD29A (RESPONSIVE TO DESICCATION 29A) and AREB2 (ABRE BINDING FACTOR 2) were determined at 0, 3, 6, 12, and 24 hpt. The relative expression levels were quantified by normalizing to ubiquitin expression level. Data are averaged ±SD (n = 4). *p ≤ .05; **p ≤ .005; ***p ≤ .0005; ****p ≤ .00005; two‐tailed t test. The experiments were independently repeated at least twice