Figure 1.

Dexamethasone‐inducible SAL1 silencing using hpRNAi pOpOff system. (a) Schematic diagram of the dex‐inducible pOpOff2(kan)‐SAL1hpRNAi plasmid vector. RB, right border; T, terminator; int, intron; nptII, kanamycin‐resistant gene; LB, left border. Three‐week‐old seedlings germinated and grown on MS supplemented with 20 μM dex were harvested for (b) SAL1 transcript quantification relative to the wild‐type Col‐0 control via qRT‐PCR and (c) 3′‐phosphoadenosine‐5′‐phosphate quantification using HPLC. At least 10 seedlings per transgenic line were pooled for each quantification. Significant differences (ANOVA, p < .05) are denoted by a, b. n = 3–4; error bars = SD