Figure 2.

Generating and testing the potential of SAL1‐silencing efficiency using hpRNAi under CaMV35S strong constitutive promoter. (a) Schematic diagram of the 35S:SAL1hpRNAi (pAgrikola as backbone) plasmid vector. RB, right border; T, terminator; int, intron; bar, BASTA‐resistant gene; LB, left border. Leaves of five‐week‐old T1 transformants were harvested for (b) SAL1 transcript quantification [n = 3 technical replicates, error bars = SD, significant differences = ANOVA, post hoc test relative to Col‐0: ***p < .001] and (c) 3′‐phosphoadenosine‐5′‐phosphate quantification [n = 1]