Figure 5.

Effects of taxol treatment on cell wall regeneration. (a) Representative images of the cellulose network in protoplasts incubated for 6, 12, or 18 hr in the presence or absence of taxol. The protoplasts were stained with Calcofluor. (b) Cellulose network (Calcofluor) and cortical microtubules (GFP‐TUB6) in UBQ10::GFP‐TUB6 protoplasts incubated for 12 hr in the presence (bottom) or absence (top) of taxol. (c–d) Total length (c), mean intensity (d), and skewness of intensity distribution (e) of the cellulose network regenerated from protoplasts incubated for 6, 12, or 18 hr in the presence or absence of taxol. Bar = 20 μm. Significance was determined by Mann–Whitney test. **p < .01, *.01 ≤ p < .05. n ≥ 104