Figure 3.

The measurement of H₂O₂ contents in wild‐type and sig2 mutants. The level of H₂O₂ was measured using Amplex Red assay and normalized to total chlorophyll content. (a) The level of H₂O₂ measured using three leaf disks from wild‐type (WT) and sig2 mutants grown on soil under white light (100 μmol m⁻² s⁻¹, long‐day condition with 8 hr dark/16 hr light cycle) at 22°C for 40 days. (b) The level of H₂O₂ measured using seedlings of WT and sig2 mutants (n = 27) grown on MS medium under constant white light (100 μmol m⁻² s⁻¹) at 22°C for 7 days. Data points in graphs represent mean (±SD, n = 4 biological repeats). Unpaired, two‐tailed Student's t test comparing sig2 mutants to Col‐0 WT, *p < .05, **p < .005, ***p < .0005, ****p < .00005