Table 2.

Concentrations of thiol peptides in the roots of OsPCS RNAi plants grown under As(III) stress

20 μmol/L As(III)	Thiol peptides (nmol/g DW)
Root	GSH	PC2	PC3	PC4	hmGSH	hmPC2	γECE	γEC
WT	1,250 ± 210	1,820 ± 340	553 ± 155	57.3 ± 15.6	2,630 ± 250	3,000 ± 180	2,020 ± 260	2,800 ± 230
PCS1 RNAi‐1	1,710 ± 270	2,230 ± 350	333 ± 87	28.9 ± 8.8	2,530 ± 140	2,600 ± 200	2,100 ± 160	2,860 ± 280
‐4	1,800 ± 440	2,350 ± 310	364 ± 111	37.2 ± 8.2	2,720 ± 110	2,870 ± 110	2,260 ± 280	3,340 ± 510
‐5	1,880 ± 450	2,450 ± 360	423 ± 110	38.8 ± 7.9	2,370 ± 320	2,650 ± 230	2,200 ± 320	3,250 ± 490
PCS2 RNAi‐1	6,680* ± 1590	1,110 ± 160	182* ± 58	17.1* ± 5.6	2,800 ± 330	861* ± 99	1,280 ± 80	3,780 ± 750
‐4	6,750* ± 1200	1,310 ± 280	214* ± 77	32.8 ± 12.9	2,960 ± 310	922* ± 170	1,330 ± 250	4,190 ± 470
‐5	4,460* ± 430	1,650 ± 80	273 ± 61	35.4 ± 7.8	2,240 ± 170	1,280* ± 230	1,430 ± 150	3,580 ± 110

The 7‐day‐old seedlings of RNAi plants and wild‐type (WT) (Nipponbare) plants were transferred to a culture solution supplemented with 20 μmol/L NaAsO₂. Three plants were bulked and harvested, and the concentration of thiol peptides in the roots was determined 8 days after transfer. Values are expressed as means ± SE from three independent experiments. An asterisk (*) shows a significant difference from WT plants (p < 0.05, Dunnett test).