Table 1.

Nicotine levels of T₁ plants of lines AOC2‐49 and JAR1‐20 after topping in a randomized complete block design experiment. (A) Analysis of variance of the RCB experiment. PCR‐positive T₁ plants of lines AOC2‐49 and JAR1‐20, and WT were randomly assigned to three blocks in the greenhouse. Each plot contained four plants of a line. The third leaves from the top of each plot were pooled 7 days after topping, and nicotine levels were determined by GC‐MS. (B) Tukey's studentized range test of nicotine level in the RCB experiment shows no significant differences between transgenic and WT lines

A
Source of variation	df	Sum of squares	Mean square	F Value	Pr > F
Model	5	60.28889672	12.05777934	2.27	.1275
Error	6	26.59059696	4.43176616
Location	3	32.12215774	10.70738591	2.42	.1648
Treatment effect	2	28.16673898	14.08336949	3.18	.1145
Corrected total	11	86.87949368

B
Tukey grouping	Mean (mg/g DW)	N	Treatment
A	9.676	4	AOC2_49
A	6.564	4	JAR1_20
A	6.303	4	WT