Figure 5.

Screening of an EMS‐mutated cell population to select strains exhibiting faster growth and higher lipid content. (a) Chlorophyll autofluorescence screening after 1.5 days of culture to sort droplets showing above‐threshold growth. (b–d) Lipid content screening after an additional 2.5 days of culture. The ratio of BODIPY to chlorophyll autofluorescence in (d) was analyzed by normalizing BODIPY peaks (b) to the corresponding chlorophyll peaks (c). Droplets showing both higher signal in BODIPY fluorescence (threshold: 0.17 V) and the ratio of BODIPY to chlorophyll fluorescence (threshold: 0.175) were sorted for further off‐chip validation