Figure 1.

mRNA detection by the MS2 system. The two depicted constructs were inserted in the same vector: GFP fused to the MS2 coat protein (MCP) with a nuclear localization signal (NLS), and the SKS14 or AT59 transcripts fused to MCP‐binding site (MCP‐bs). A control construct termed MS2 encodes the GFP‐MCP chimera and no target mRNA. The three constructs SKS14‐MS2,AT59‐MS2, and MS2 were under the control of the pollen‐specific promoter LAT52. LB and RB, left and right borders of the translocation cassette, respectively