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. 2017 Oct 16;1(4):e00012. doi: 10.1002/pld3.12

Figure 2.

Figure 2

Arabidopsis SKS14 and AT59 mRNAs form cytoplasmic granules in mature pollen grains. (a) Confocal images of a representative pollen grain from the control MS2 line. A mask was applied to eliminate vegetative nucleus and facilitate cytoplasm visualization. (b‐e) Representative images of two independent SKS14‐MS2 (b and c) and two AT59‐MS2 lines (d and e). In the left panels, white arrowheads show examples of cytoplasmic granules identified by the MATLAB script while empty arrowheads show cytoplasmic aggregates that were not detected by the MATLAB script. Right panels, DIC images. Size bar, 5 μm. (f) Quantification of cytoplasmic granules. Each point corresponds to the mean value of an independent sample that included 20 pollen grains. The media and standard error for each transgenic line are shown. Statistical significance (Mann–Whitney test) relative to the control line MS2 3‐1 is indicated (***p < .001 and **p < .01)