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. 2017 Oct 16;1(4):e00012. doi: 10.1002/pld3.12

Figure 5.

Figure 5

SKS14‐RFP protein is expressed from early stages of germination and localizes at the margins of pollen tubes. Representative images of S2 line pollen grains expressing SKS14‐RFP protein (left panels). Right panels, DIC images. Size bars, 5 μm for early and late stages (a and b) and 15 μm for the pollen tube (c). (d) A 5X magnification shows localization at the tip (Size bar, 5 μm). (e) Quantification of SKS14 mRNA cytoplasmic granules per pollen grain compared to a MS2 control (MS2 3‐1). ES and LS, early and late stages of pollen germination, respectively. Each point represents the mean value of independent samples including 9‐10 pollen grains. Lines link data from paired samples (same experiment). Statistically different values are indicated (***p < .001 and **p < .01). The p‐value for the ES‐MS2/LS‐MS2 pair was 0.99, and for the LS‐MS2/LS‐S13 pair, 0.48 (two‐way ANOVA randomized block, Bonferroni post‐test)