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. 2018 Apr 25;2(4):e00056. doi: 10.1002/pld3.56

Figure 5.

Figure 5

Validation of RNA‐Seq expression measurements by qPCR in three tef genotypes. Aquaporin (a), bifunctional nuclease 2‐like gene (b), cinnamoyl reductase‐like gene (c), and granule‐bound starch synthase (d) were regulated under waterlogging conditions using RNA‐Seq. Expression measurements for these four transcripts were verified with qPCR, using either CYP or PP2A as reference genes. Three replicates were used for each genotype, condition, and transcript