Table 1.
Experiment type | Genotypes | Number of plants of each genotype | Tissue | Age of plant during treatment | Evaluation methods | Parameters measured | Stage Figure or Table | |
---|---|---|---|---|---|---|---|---|
1 | Growth 1 | Alba, Quncho, Tsedey | 18 | Roots, shoots | From 4 to 28 days | Measure shoot length in pots | Shoot length |
Early stage Figure S1 |
2 | Growth 2 | Quncho, Tsedey | 66 | Roots, shoots | From 4 to 28 days | Harvest 4 controls and 4 waterlogged at 2, 4, 7, 9, 11, 16, and 24 days after treatment | Root and shoot length, dry weight, cross section |
Early stage Figures 1 and S6 Tables 2, S1, S2 |
3 | Root growth 1 | Alba, Quncho, Tsedey | 2 | Roots | From 4 to 22 days | Visualization with rhizotron | Root growth |
Early stage Figure S2 |
4 | Root growth 2 | Wheat, maize, Alba, Tsedey | 2 | Roots, shoots | From 7 to 35 days | Visualization with rhizotron | Root growth |
Early stage Figures S3 (wheat) and S4 (maize) |
5 | Physiology | Tsedey | Shoots | From 19 to 28 days |
Porometer SPAD |
Chlorophyll a and b, carotenoid, stomatal conductance |
Tillering stage Figure 2 |
|
6 | Cross sections | Alba, Quncho, Tsedey | 21 | Roots | From 19 to 28 days | Count adventitious roots, cross sections |
Number of adventitious roots Aerenchyma size |
Tillering stage Figures 3, 4 and S5 Table S3 |
7 | qPCR | Alba, Quncho, Tsedey | Shoots | From 19 to 28 days | Microscopy, qPCR | Differential gene expression |
Tillering stage Figure 5 Table S6 |
|
8 | RNA‐Seq | Tsedey | Shoots | From 19 to 28 days | RNA‐Seq | Differential gene expression |
Tillering stage Figures S7, S8, S9, S10, S11, and S12 Tables 3, 4, 5, S4, and S5 |