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. 2019 May 9;14(5):e0216815. doi: 10.1371/journal.pone.0216815

Fig 2. Reprogramming Vγ9Vδ2 T cells into γδ T-iPSCs.

Fig 2

(a) A schematic of reprogramming strategy #3 that uses γδ T cell-enriched cell clump population and nucleofection to generate γδ T-iPSCs. (b-c) Morphology (b) and phenotype (c) of PBMCs cultured with zoledronic acid and IL-2. The numbers in the dot plots indicate the % of CD3+γδ TCR+ cells. (d) Morphology of three iPSC lines generated with reprogramming strategy #3. (e) Identification of γδ T-iPSC line using TCRG gene clonality assay. The yellow arrow indicates positive amplified product. (f) Detection of CD3 and γδ TCR expression in three iPSC lines by flow cytometry. (g) A result summary of γδ T-iPSC generation using reprogramming strategy #3.